Special Seminar | Optimizing the photon budget: 4D imaging of biological dynamics | Kevin Keomanee-Dizon | University of Southern California

In the last few decades, there have been transformative advances in optical microscopy that now offers us the ability to observe the organization and dynamics of biological systems at high resolution in space and time.  Such 4D imaging, however, is challenged by unavoidable tradeoffs between spatial resolution, acquisition speed, penetration depth, and noninvasiveness in the face of a limited photon budget. In this talk, I will describe the development of three microscope technologies that optimize the photon budget in different ways: one- and two-photon light-sheet microscopy for 4D dynamic visualization of living systems; wavefront shaping for extended focus imaging; and light fields for synchronous recording of volumes spanning hundreds of microns, at rates limited only by the detector and available signal. In each case I will present collaborative applications in capturing some of life’s most fascinating phenomena, from bacterial flows to neural activity throughout the entire brain of a behaving animal.


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