Microscopy has played important roles in revealing new insight in diverse fields of research ranging from physics to biology. It likewise is an important component of controlling nano-scale structures in the semiconductor industry. I will trace my own path of adapting various forms of scanned probe microscopy to image vortices, electrons, and quantum wells for basic physics research. A decade in industry taught the methodology and importance of high throughput imaging and massive data processing, (the Giga to complement the Nano). A lucky break allowed me and former Bell Labs colleague, Eric Betzig, to apply such lessons to biology with the innovation of photo-activated localization microscopy PALM and extensions. Recently electron microscopy where innovations in 3D high volume imaging are enabling neural tissue and cellular imaging with volume scales and resolution not previously accessed. How to extend and harness such data sets and translate them to biology remains a major challenge. **New Location: McDonnell A02**