We have recently demonstrated a technology using sequential hybridization and single molecule FISH to multiplex a large number of mRNA molecules directly in single cells in complex tissue samples. mRNAs in cells are barcoded by sequential rounds of hybridization, imaging, and probe stripping. The number of barcodes available with this approach scales as F^N, where F is the number of distinct fluorophores and N is the number of hybridization rounds. We call this method seqFISH and it is conceptually akin to “sequencing” mRNAs directly in cells by FISH. We will discuss application of this technology to brain sections, embryos, and human tissues.
Lunch @ 11:45, seminar 12-1:00